New preprints!

We are very excited to share two new preprints from our lab, both out today!

The first project, led by grad student Mike Van, discusses using nanobodies to recruit endogeneous chromatin regulators at a target locus to control gene expression and epigenetic memory. Check it out here!

The second, led by grad student Josh Tycko, describes HT-recruit, a method for testing thousands of protein domains in human cells, and some of the results we got applying it to transcriptional effectors. Check it out here!

Black Lives Matter

In light of recent events, we would like to take a moment to acknowledge the pain felt by Black communities due to the murder of Nina Pop, Tony McDade, Breonna Taylor, George Floyd, and Ahmaud Arbery. They are but the latest victims in a long line of stolen Black lives. 

We have taken time to reflect on what we as a lab can do to help fight against anti-Black racism and discrimination in all its forms. We are committed to using our resources and our privilege to lift those who are ignored, demeaned, and mistreated by a society that fails to appropriately value and treasure the lives of its minorities. 

Additionally, we would like to invite any individuals from underrepresented backgrounds to reach out to us if interested in a research position, whether that be as a research assistant, graduate student, or postdoc. We are an interdisciplinary group and that puts us in the habit of helping each other, since all of us come with gaps in certain areas by definition. Our commitment to you is to foster an inclusive environment and provide each person with the resources and support they need to thrive as a scientist and human being. 

2019 Bintu Lab Retreat

We wrapped up the summer by going up North to wine country.

We had an amazing time wine-tasting, hanging out by the Russian River, and discussing science. And this time we actually remembered to take some pictures:

Awesome brunch at the house in Ukiah that we rented for the night:

Postdoc Positions Available

The lab of Dr. Lacra Bintu employs single-cell methods, synthetic biology, and mathematical modeling to understand chromatin and gene regulation in a quantitative manner. The lab is part of Stanford University’s Bioengineering Department, which is joint between the Schools of Engineering and Medicine. Postdocs will have access to considerable resources for cell biology, fluorescence imaging, epigenomics, and computation in the lab as well as participate in a stimulating, productive research community. Positions are funded and provide competitive salaries for living in the beautiful northern California area.

Postdoc positions are available in the following areas:

  • single-cell, multiplexed detection of chromatin modifications
  • high-throughput synthetic manipulations of chromatin
  • the role of chromatin in the innate immune response, including natural killer cells

Projects are not restricted to these topics; if you are interested in chromatin and gene regulation and enjoy quantitative puzzles, apply to do fun science with us!

Postdoc candidates, please send a CV, including names and contact information for 3 references. Additionally, please provide a brief cover letter describing your previous experience, career goals, proposed start date, and an informal description of your general scientific interests. Please email all documents in PDF format to: lbintu@stanford.edu.

(Position filled) Job Opening – Research Assistant for Mammalian Cell Engineering

An immediate opening is available for a full-time (1-2 year commitment) research assistant in the laboratory of Dr. Lacra Bintu in the Department of Bioengineering at Stanford University. The Bintu Lab uses systems and synthetic biology approaches to characterize the dynamics of gene and chromatin regulation in mammalian cells. This is an opportunity to work on a project investigating chromatin dynamics in different mammalian cells. Techniques used include engineering of mammalian cell lines, flow cytometry analysis of mammalian cells, and time-lapse microscopy movies of mammalian cells. The position will involve close collaboration with an interdisciplinary team. This is an excellent training opportunity for anyone interested in attending graduate school or medical school. Cover Letter should indicate earliest possible joining date. Stanford is an equal opportunity employer.

CORE DUTIES*:

  • Plan and perform experiments in support of research projects in lab
  • Interpret and perform basic analysis of results
  • Review literature to remain current with new procedures and related research
  • Contribute to creation and modification of procedures and protocols in collaboration with graduate students and postdoctoral fellows
  • Maintain detailed records of experiments and outcomes
  • Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals
  • Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed

* Other duties may also be assigned

QUALIFICATIONS:

  • Strong academic record in molecular biology and synthetic biology
  • Experience in molecular lab environment, specifically cloning and sterile tissue culture
  • Attention to detail and critical thinking
  • Ability to following detailed instructions and maintain accurate records
  • Excellent communication and organizational skills
  • Excellent interpersonal skills and ability to work with a diverse group of people

For more details, contact Lacra at lbintu [at] gmail [dot] com.

New students and MIRA funding

We are so glad to welcome our new undergraduate student, Michael Herschl (Bioengineering) and our new graduate students Adi Mukund (MD/PhD) and Connor Ludwig (Bioengineering) to the lab!

In other news, the lab was awarded the MIRA R35 from NIGMS. Congratulations all!

First Bintu Lab publication

We wrote a review article covering the recent developments at the intersection of single-cell measurements, mammalian synthetic biology manipulations, and mathematical models of gene regulation.

PDF: Tycko & Van, 2017, Current Opinion in Biomedical Engineering

Here we review recent work that has taken promising initial steps towards the kind of quantitative, single-cell gene regulation framework that will enable us to understand, predict, and design gene regulatory systems in mammalian cells.

We had a great time diving into the literature and writing out our view of the field at this exciting moment. Enjoy this early look at what the Bintu lab is thinking about.

The lab is growing!

Our three new graduate students bring in diverse backgrounds and research skills:

Mike Van (Biology)

Josh Tycko (Genetics)

Sarah Lensch (Bioengineering)

Welcome to the lab everybody!

Lab BBQ with new students, rotation students, and significant others. This time we remembered to take a photo for the website 🙂

The new lab space is up and running

The incubators are shaking, the scope is imaging, the thermocyclers are cycling, the massive Goodsell poster of the cell is hung on the wall – we are all moved in!

Thank you to the formidable efforts of lab manager Rhonda DiGiusto and building manager Patrick Carlson to create our beautiful space.

We’re sharing the space with Possu Huang’s lab of protein engineers.